A novel compound Lup-20 (29)-ene-3α,6β-diol identified in petroleum ether extract of diospyros melanoxylon Roxb. leaves and to reveal its antidiabetic activity in rats

Anil Kumar Sharma; Tek Chand Sharma; Ruchi Singh; Pratibha Payal; Rajnish Gupta; Mahesh Chandra Sharma

Articles

Abstract

Pharmacognosy Magazine,2018,14,55s,s245-s248.

DOI:10.4103/pm.pm_429_17

Published:June 2018

Type:Original Article

Authors:

Author(s) affiliations:

Anil Kumar Sharma¹, Tek Chand Sharma², Ruchi Singh³, Pratibha Payal², Rajnish Gupta⁴, Mahesh Chandra Sharma²
¹ Department of Chemistry, Jagan Nath University, Jaipur, Rajasthan, India
² Department of Chemistry, Natural Products Laboratory, University of Rajasthan, Jaipur, Rajasthan, India
³ Department of Chemistry, Natural Products Laboratory, University of Rajasthan; Department of Chemistry, The IIS University, Jaipur, Rajasthan, India
⁴ Department of Zoology, University of Rajasthan, Jaipur, Rajasthan; Department of Neurobehavioral Toxicology, ICMR-NIOH, Ahmedabad, Gujarat, India

Abstract:

Background: Diospyros melanoxylon Roxb. belongs to the family Ebenaceae and its leaves show diuretic, carminative, laxative, and styptic bioactivities. Objective: The objective of this study was to give a direction for future investigators to carry out research on phytochemistry and develop a potent antidiabetic agent. Materials and Methods: Petroleum ether extract of D. melanoxylon Roxb. leaves was chromatographed over silica gel column. Isolated compounds were elucidated on the basis of different spectroscopic techniques (mass spectrometry, infrared, 1H, 13C nuclear magnetic resonance, and distortionless enhancement by polarization transfer). Antidiabetic activity of lup-20 (29)-ene-3α,6 β-diol was evaluated in streptozotocin diabetic rats. Results: A novel compound, lup-20 (29)-ene-3α,6 β-diol (6), was identified along with 11 known natural compounds, namely, lupeol (1), ceryl alcohol (2), octacosanol-1 (3), hentriacontanol-1 (4), β-sitosterol (5), diospyrin (7), 3α-methoxydiospyrin (8), betulin (9), ursolic acid (10), betulinic acid (11), and oleanolic acid (12). Further administration of lup-20 (29)-ene-3α,6 β-diol significantly improved the diabetes-induced oxidative stress. Conclusion: Investigation of this novel compound plays an important role in the field of drug development.