Comparative study on the antitumour activity of 20(R)-ginsenoside Rh2 and 20(S)-ginsenoside Rh2 from Panax ginseng against Non-small cell lung cancer in vitro

Xinze Liu; Xin Jin; Xinmin Wu; Lin Feng; Hongmei Yang; KaiJing Sun; Xue Yang; Ruina Lv; Yuhe Ren; Mengle Xie; Jianhua Lv; Lan Yao; Daiyao Liu; Wei Wu; Shuying Liu; Changbao Chen; Xilin Wan

Articles

Abstract

Pharmacognosy Magazine,2022,18,80,1011-1024.

DOI:10.4103/pm.pm_583_21

Published:November 2022

Type:Original Article

Authors:

Author(s) affiliations:

Xinze Liu¹, Xin Jin², Xinmin Wu³, Lin Feng¹, Hongmei Yang¹, KaiJing Sun¹, Xue Yang⁴, Ruina Lv⁵, Yuhe Ren⁶, Mengle Xie², Jianhua Lv⁵, Lan Yao⁵, Daiyao Liu⁵, Wei Wu¹, Shuying Liu¹, Changbao Chen¹, Xilin Wan⁷
¹Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China
²Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, China
³Department of Neurosurgery, First Hospital of Jilin University, Changchun, China
⁴Cardiovascular Medicine and Cardiac Rehabilitation Center, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, China
⁵International Cooperation Research Center of China for New Germplasm and Breeding of Edible Mushrooms, Jilin Agricultural University, Changchun, China
⁶Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, China

Abstract:

Background: Non-small cell lung cancer (NSCLC) is often harmful to human health. It is common in people who smoke or smoke passively, and the affected people are older and find it difficult to recover. The main treatment methods are surgery, radiotherapy and chemotherapy. This paper aims to study the effects of different configurations of ginsenoside Rh2 on NSCLC 95D and NCI-H460 cells. To provide clinical value for the treatment of NSCLC. Materials and Methods: CCK-8 was used to determine the proliferation of 95D and NCI-H460 cells with different concentrations of ginsenoside Rh2 (0, 0.05, 0.1 and 0.2 mg/mL), and IC₅₀ was calculated. Cell cycle and apoptosis were detected by immunofluorescence staining and flow cytometry. Results: 20(R)-ginsenoside-Rh2 and 20(S)-ginsenoside-Rh2 inhibited the proliferation and colony formation of NSCLC 95D and NCI-H460 cells induced cell cycle arrest in a time and dose-dependent manner in G1/S and promote apoptosis. Conclusion: Ginsenoside Rh2 induces antitumour activity by inhibiting proliferation and colony formation of 95D and NCI-H460 cells and inducing cell cycle arrest and apoptosis. Comparing the two configurations of ginsenoside Rh2, the effect of 20(R)-ginsenoside-Rh2 is stronger.