Phytochemical analysis, antipropulsive and antilymphoma activities of leaves extract of Annona cherimola miller

Fernando Calzada; Miguel Valdés; Verenice Itzel Merlin; Claudia Velázquez; Elizabeth Barbosa; Marco Chávez-Soto; Emmanuel Pina-Jiménez; Rosa Maria Ordoñez-Razo; Normand García-Hernández

Articles

Abstract

Pharmacognosy Magazine,2020,16,69,317-320.

DOI:10.4103/pm.pm_462_19

Published:June 2020

Type:Original Article

Authors:

Author(s) affiliations:

Fernando Calzada¹, Miguel Valdés¹, Verenice Itzel Merlin¹, Claudia Velázquez², Elizabeth Barbosa³, Marco Chávez-Soto¹, Emmanuel Pina-Jiménez⁴, Rosa Maria Ordoñez-Razo⁴, Normand García-Hernández⁴
¹ Unidad de Investigación Médica en Farmacología, Unidad Médica de Alta Especialidad, Hospital de Especialidades-2° Piso CORSE Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Cd México, Mexico
² Área Académica de Farmacia, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, San Agustin Tlaxiaca, Pachuca, Hidalgo, Mexico
³ Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, Cd Mexico, Mexico
⁴ Unidad de Investigación Médica en Genética Humana Unidad Médica de Alta Especialidad, Hospital de Pediatría. Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Cd Mexico, Mexico

Abstract:

Background: Diarrhea and cancer are significant health problems worldwide. Annona cherimola Mill. (Annonaceae) is a fruit tree extensively used in Mexican traditional medicine for the treatment of diarrhea and cancer. Objectives: The present work reports the identification of flavonol glycosides present in the ethanol crude extract using high-performance liquid chromatography with diode array detection (HPLC-DAD). Materials and Methods: The identification of flavonoids was made by comparing their retention times and ultraviolet spectra with those standards. The crude extract, dichloromethane, and aqueous residual (AR) fractions were tested for their antipropulsive and antilymphoma activities. Results: The results revealed high levels of three flavonoid glycosides (rutin, nicotiflorin, and narcissin), especially rutin (24.5 mg/g of extract). The antipropulsive and antilymphoma activities of the leaf extract and subsequent fractions showed that the ethanol extract of the leaves of A. cherimola is cursive and AR fraction exhibited the best biological effects in both assays. Conclusion: The HPLC-DAD method used in this work enables the determination of rutin as a major flavonol glycoside of the leaves of A. cherimola . In addition, it can be suggested that rutin may play an important role in the biological properties of A. cherimola.