Effect of ethanolic extract of Carica papaya Leaves and their cytotoxicity and apoptotic potential in human ovarian cancer cell lines- PA-1

Introduction: The aim of the present study is to investigate the efficacy of Carica papaya ethanolic extract for antiproliferative, cytotoxicity, apoptotic, cell cycle blockade and wound healing in the ovarian cancer PA-1 cell lines. Materials and Methods: PA-1 cells were treated without the sample (act as a control) and with the sample for the above assay as per the described protocol. Results: The end result antiproliferative showed that PA-1 cell viability decreases in a concentration dependent manner and the growth inhibitory effect (IC50) values are obtained at a concentration of 100 μg. The increase in number of apoptotic cell was observed and after treating with PA-1 cells the sample with double staining methods. G2/M phase of the cell cycle was significantly blocked by the test sample followed by the S phase in a negligible manner. And in vitro cell wound closure or contracture was not significant when compared the sample against control group. Conclusion: C. papaya ethanolic extract showed to possess excellent cytotoxic effect through inducing apoptosis especially causing arrest at the G2/M phase of the cell cycle.