The effects of extraction method on recovery rutin from Calendula officinalis L. (Asteraceae)

Frederico Severino Martins; Edemilson Cardoso da Conceição; Elane Sousa Bandeira; José Carréra Silva; Roseane Maria Ribeiro Costa

Articles

Abstract

Pharmacognosy Magazine,2014,10,39s,s569-s573.

DOI:10.4103/0973-1296.139785

Published:August 2014

Type:Original Article

Authors:

Author(s) affiliations:

Frederico Severino Martins¹, Edemilson Cardoso da Conceição², Elane Sousa Bandeira³, José Carréra Silva³, Roseane Maria Ribeiro Costa³
¹ Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil
² Department of Pharmaceutical Sciences, Faculty of Pharmacy Research, Federal University of Goiás, Brazil
³ Department of Pharmaceutical Sciences, College of Pharmacy, Federal University of Para, Pará, Brazil

Abstract:

Background: Calendula officinalis L. (Asteraceae) is a Mediterranean specie, but in Europe and America it is cultivated for ornamental or medicinal purposes. This species is widely used for presenting activities, antiinflammatory antibacterial and antioxidant. However the therapeutic action is linked to the amount of assets of the extracted raw material. The extraction method of bioactive compounds is an important step in the manufacturing of herbal medicines, because secondary metabolites with therapeutic potential are usually found in small quantities in plant materials. Objective: Due the medical and commercial importance of C. officinalis, this study aimed to evaluate the impact of the extraction method on the quality of herbal extract and optimize the extraction of rutin from C. officinalis. Materials and Methods : The extraction of rutin was performed by ultrasound and shaker and the optimized conditions were determined by response surface methodology. Results: The results of ultrasound extraction assisted (UEA) and maceration dynamic (MD) showed that rutin yield ranged from 0.218 to 2.28% (w/w) when extract by ultrasound and 0.1-1.44% by MD. The optimal extraction condition for rutin (2.48% to UEA or 1.46% to MD) from C. officinalis by UEA or MD were a 19-22 min extraction, ethanol: water ratio of 35-40% and 0.05-0.056 mg/mL to raw material: solvent ratio. Conclusion: The UEA is more efficient to extraction rutin.