Content determination of the flavonoids in the different parts and different species of Abelmoschus esculentus L. by reversed phase-high performance liquid chromatograph and colorimetric method

Yin Lin; Min-feng Lu; Hai-bing Liao; Yu-Xian Li; Wei Han; Ke Yuan

Articles

Abstract

Pharmacognosy Magazine,2014,10,39,278-284.

DOI:10.4103/0973-1296.137368

Published:July 2014

Type:Original Article

Authors:

Author(s) affiliations:

Yin Lin¹, Min-feng Lu², Hai-bing Liao², Yu-xian Li³, Wei Han¹, Ke Yuan²
¹ School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
² Zhejiang Agriculture and Forestry University, Lin'an 311300, Zhejiang, China
³ College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, Henan, China

Abstract:

Background: This research will establish the ultraviolet colorimetric method to determine the total flavonoid content in different species and different parts of Abelmoschus esculentus L. Materials and Methods: We establish the reversed-phase high-performance liquid chromatograph (RP-HPLC) method to determine the content of the three flavonoid glycosides in different species and different parts of the A. esculentus. Adopt the NaNO ₂ -Al (NO ₃ ) ₃ -NaOH colorimetric method to determine the total flavonoid content; at the same time, adopt the RP-HPLC method to determine the contents of the three flavonoid glycosides. Using the methods of ultraviolet colorimetry and RP-HPLC, we determined and analyzed the total flavonoid content and the content of the three flavonoid glycosides in different species and different parts of A. esculentus. Results: There are great distribution differences of the total flavonoids and the three flavonoid glycosides in different species and parts of A. esculentus. Among them, the content of the effective constituents in the flower is relatively high, next is in the fruit. In the different species of A. esculentus, the content of the flavonoids of finger relatively high. The HPLC method established in this research is simple and convenient and its results are accurate and reliable. In addition, it has a very good repeatability. Conclusion: The results provided the reference data for the medicinal use of A. esculentus and it can be used in quality analyzing of its effective constituents.