Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze

Xinhui Tang; Lifang Yan; Jing Gao; Haiyan Ge; Haidong Yang; Na Lin

Articles

Abstract

Pharmacognosy Magazine,2011,7,27,186-192.

DOI:10.4103/0973-1296.84225

Published:August 2011

Type:Original Article

Authors:

Author(s) affiliations:

Xinhui Tang¹, Lifang Yan², Jing Gao³, Haiyan Ge³, Haidong Yang³, Na Lin³
¹Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002, People's Republic of China
²Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002; School of Pharmacy, Jiangsu University, Zhenjiang - 212013, People's Republic of China
³School of Pharmacy, Jiangsu University, Zhenjiang - 212013, People's Republic of China

Abstract:

Objective: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) Materials and Methods : One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro. Results: According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity. Conclusion: One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.