Antioxidant and hepatoprotective potential of Swaras and Hima extracts of Tinospora cordifolia and Boerhavia diffusa in Swiss albino mice

Amit Kaushik; Athar Husain; Harshika Awasthi; Dewasya Pratap Singh; Raziuddin Khan; Dayanandan Mani

Articles

Abstract

Pharmacognosy Magazine,2017,13,51s,s658-s662.

DOI:10.4103/pm.pm_448_16

Published:October 2017

Type:Original Article

Authors:

Author(s) affiliations:

Amit Kaushik¹, Athar Husain¹, Harshika Awasthi², Dewasya Pratap Singh², Raziuddin Khan³, Dayanandan Mani²
¹Herbal Medicinal Products Department, CSIR-Central Institute of Medicinal and Aromatic Plants; Mahatma Gandhi Institute of Pharmacy, Lucknow, Uttar Pradesh, India
²Herbal Medicinal Products Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India
³Mahatma Gandhi Institute of Pharmacy, Lucknow, Uttar Pradesh, India

Abstract:

Background: In Ayurveda, five basic extraction procedures are mentioned in order of their decreasing potency. Swaras is considered as the most potent followed by, kalka, kwatha, fanta and hima. Objective: Present study was carried out to investigate the antioxidant and hepatoprotective potential of swaras and hima extracts of T.cordifolia and B. diffusa. Materials and Methods: Swaras and hima extracts of T. cordifolia and B. diffusa were prepared. Phytochemical screening and in vitro antioxidant activities was carried out using standard methods. Hepatoprotective efficacy of extracts were carried out in Swiss albino mice using paracetamol induced hepatotoxicity. Animals were administered with swaras and hima extracts of both plants at 200 mg/kg BW dose for 7 days and on 8^th day hepatotoxicity was induced by intraperitoneal injection of paracetamol at 500 mg/kg BW. The degree of liver protection was determined by measuring the levels of liver enzymes followed by histopathology. Results and Discussion: The results of phytochemical, antioxidant and hepatoprotective activities showed that there were no significant difference between swaras and hima extracts. Both the extract of T. cordifolia were equally potent in reducing SGOT (P < 0.01) and ALP level (P < 0.001). Similar effects were observed with the Swaras and hima extracts of B. diffusa. Both the extracts reduced SGOT and ALP (P < 0.01). Histopathological findings among all the extracts were also more or less similar in lowering the paracetamol mitigated necrosis. Conclusion: The present study suggested that T. cordifolia and B. diffusa possess potential hepatoprotective activity irrespective of the extraction procedure.