In vitro protoscolicidal effects of Cinnamomum zeylanicum essential oil and its toxicity in mice

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Abstract
Pharmacognosy Magazine,2017,13,51s,s652-s657.
Published:October 2017
Type:Original Article
Authors:
Author(s) affiliations:

Hossein Mahmoudvand1, Hormoz Mahmoudvand2, Razieh Tavakoli Oliaee3, Amir Tavakoli Kareshk3, Seyed Reza Mirbadie4, Mohammad Reza Aflatoonian5
1Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran
3Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran
4Department of Laboratory Sciences, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
5Bam University of Medical Sciences, Bam; Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran

Abstract:

Background: This study investigates the scolicidal effects of Cinnamomum zeylanicum essential oil against the protoscoleces of hydatid cysts and its toxicity in the mice model. Materials and Methods: Gas chromatography/mass spectroscopy analyses were used to identify the constituents of essential oil. Protoscoleces were treated with different concentrations of the essential oil (6.25–100 μL/mL) in each test tube for 5–30 min. The viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oil (0.5–4 mL/kg). Results: The main components were found to be cinnamaldehyde (91.8%), ρ metoxicinamate (1.57%), and α pinene (1.25%). Findings indicate that C. zeylanicum essential oil with the concentrations of 100 and 50 μL/mL killed 100% of protoscoleces after 5 min of exposure. Also, the lower concentrations of C. zeylanicum essential oil motivated a late protoscolicidal effect. The LD50value of intraperitoneal injection of C. zeylanicum essential oil was 2.07 mL/kg body weight after 48 h, and the maximum nonfatal dose was 1.52 mL/kg body weight. The results also showed that there was no significant toxicity following oral administration of C. zeylanicum essential oil for 2 weeks. Conclusion: The results exhibited the favorable scolicidal activity of C. zeylanicum, which could be applied as a natural scolicidal agent in hydatid cyst surgery.

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