Naringenin reduced migration in osteosarcoma cells through downregulation of matrix metalloproteinase-2 and matrix metalloproteinase-9 and snail

Yuh-Ming Chang; Ming-Cheng Lin; Ling-Zong Hong; Pei-Ni Chen; Yu-Hsin Tsai; You-Cheng Hseu; Ke-Min Chen; Li-Sung Hsu; Ting-Hsien Kao

Articles

Abstract

Pharmacognosy Magazine,2020,16,71,620-624.

DOI:10.4103/pm.pm_31_20

Published:October 2020

Type:Original Article

Authors:

Author(s) affiliations:

Yuh-Ming Chang¹, Ming-Cheng Lin², Ling-Zong Hong³, Pei-Ni Chen⁴, Yu-Hsin Tsai⁴, You-Cheng Hseu⁵, Ke-Min Chen⁶, Li-Sung Hsu⁷, Ting-Hsien Kao⁸
¹ Department of Neurology, Division of Internal Medicine, Hsinchu Mackay Memorial Hospital, Hsinchu; Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan
² Department of Internal Medicinel; School of Medicine, Chung Shan Medical University, Taichung, Taiwan
³ Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan
⁴ Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan
⁵ Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University; Department of Health and Nutrition Biotechnology, Asia University; Chinese Medicine Research Center, China Medical University, Taichung, Taiwan
⁶ Department of Parasitology, School of Medicine, Chung Shan Medical University, Taichung, Taiwan
⁷ Institute of Biochemistry, Microbiology, and Immunology; Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan
⁸ Division of Functional Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung; Department of Neurosurgery, Lin Shin Medical Corporation Lin Shin Hospital, Taichung, Taiwan

Abstract:

Background: Osteosarcoma is one of the most malignant cancers in children. Naringenin exhibits several cellular functions. Objectives: In this study, we investigate the effects of naringenin on osteosarcoma. Materials and Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to detect the cell viability. Zymography assay and transwell assay were used to measure the matrix metalloproteinase (MMP) activity and migration ability. Western blot analysis was used to determine the expression of migration-related proteins. Results: No overt alternation of cytotoxicity in response to different concentrations of naringenin for 24 h was found by MTT assay. MMP-2 and MMP-9 activities and expression were significantly repressed by naringenin in a dose-dependent manner, as evidenced by zymography and Western blot analysis. Naringenin dose dependently reduced the expression of mesenchymal marker Snail. Conclusion: These results indicate that naringenin exhibited antimigration property through inhibition of MMP-2 and MMP-9 and downregulation of Snail expression. Thus, naringenin may be a potential inhibitor of metastasis of osteosarcoma.

Keywords:Matrix metalloproteinase, Migration, naringenin, osteosarcoma, Snail