Background: Among the various metal nanoparticles, plant extract-based silver nanoparticles are gradually getting preference because the bioreduction potential of plant extracts is much faster for silver ions. The silver nanoparticles have the bactericidal activity and have shown to possess anticancer potential for developing effective and safe novel therapeutic agents. Objectives: The objective of the study was to synthesize Curcuma longa silver nanoparticles for the induction of anticancer activity in breast cancer cell. Materials and Methods: The 20 ml and 10 ml of C. longa stock extract were mixed with 10 ml and 10 μl of AgNO3solution (A) 1 mM and (B) 1M, respectively; reduction process initiates the formation of nanoparticles as detected by color change of AgNO3from brown to dark brown; thereafter, characterization done by ultraviolet–visible spectrophotometer, Scanning electron microscope, and transmission electron microscopy. Results: The synthesized and characterized C. longa silver nanoparticles induce targeted anticancer activity in breast cancer cells.