Therapeutic effect of total saponins from Dioscorea nipponica makino on gouty arthritis based on the NF-κB signal pathway: An In vitro study

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Abstract
Pharmacognosy Magazine,2016,12,47,235-240.
Published:July 2016
Type:Original Article
Authors:
Author(s) affiliations:

Qi Zhou1, Shumin Liu2, Donghua Yua1, Ning Zhang1
1 Research Institute of Chinese Medicine, Harbin 150040, PR China
2 Technological Innovation Team of Basic Theory Study Research of Institution of Higher Education in Heilongjiang Province, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China

Abstract:

Objective: Dioscorea nipponica Makino is one of the most common used traditional Chinese drugs which are used to treat gouty arthritis (GA). Nuclear factor-κB (NF-κB) pathway plays an important role during this process. In the present study, we investigated the effects of total saponins from D. nipponica Makino (TDN) on NF-κB pathway in interleukin-1β (IL-1β) induced fibroblast-like synoviocytes (FLS). Materials and Methods: FLS were divided into three groups: Normal group, model group, which was given 10 μg/L IL-1β to induce the proliferation, and TDN group (10 μg/L IL-1β +100 μg/L TDN). 1 h, 24 h, 48 h, and 72 h after treating, immune fluorescence method was used to detect the cell location of NF-κB p65. Electrophoretic mobility shift assay was used to detect the activation of NF-κB p65. Western blot method was used to detect the protein expressions of NF-κB p65, IκBα, and p-IκBα. Results: TDN could inhibit the activation and transfer of NF-κB p65. As time went on, the expression of NF-κB p65 in the cytoplasm was decreased while it was increased in the nucleus. The expression of p-IκBα was increased, whereas the expression of IκBα was not changed. TDN could regulate these abnormal expressions. Conclusion: TDN may treat GA by regulating NF-κB signal pathway.

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