Multi-component analysis in sun-dried and sulfur-fumigated Angelicae Sinensis Radix by single marker quantitation and chemometric discrimination

Yajing Lou; Hao Cai; Xiao Liu; Gang Cao; Sicong Tu; Songlin Li; Xiaoqing Ma; Kunming Qin; Baochang Cai

Articles

Abstract

Pharmacognosy Magazine,2014,10,37s,s189-s197.

DOI:10.4103/0973-1296.127374

Published:February 2014

Type:Original Article

Authors:

Author(s) affiliations:

Yajing Lou¹, Hao Cai², Xiao Liu², Gang Cao³, Sicong Tu⁴, Songlin Li⁵, Xiaoqing Ma¹, Kunming Qin¹, Baochang Cai⁶
¹ Department of Chinese Materia Medica, College of Pharmacy; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, China
² Department of Chinese Materia Medica, College of Pharmacy; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing; National First Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing, China
³ Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing; National First Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing; Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China
⁴ Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia
⁵ Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Chinese Medicine, Nanjing, China
⁶ Department of Chinese Materia Medica, College of Pharmacy; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing; National First Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing; Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou, China

Abstract:

Background: A new method has been developed for the simultaneous determination of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix before and after sulfur-fumigation using quantitative analysis of multi-components by a single marker (QAMS). Materials and Methods: The feasibility and accuracy of QAMS were checked by the external standard method, and various high-performance liquid chromatographic instruments and chromatographic conditions were investigated to verify its applicability. Using ferulic acid as the internal reference substance, and the contents of senkyunolide A and Z-ligustilide were calculated according to relative correction factors by high-performance liquid chromatography. Meanwhile, the influence of sulfur-fumigation on these chemical components in Angelicae Sinensis Radix were evaluated and discriminated by chromatographic fingerprint and chemometrics. Results: There was no significant difference observed between the QAMS method and the external standard method. Furthermore, sulfur-fumigation reduced the contents of ferulic acid, senkyunolide A, and Z-ligustilide in Angelicae Sinensis Radix by some degree, and the sun-drying and sulfur-fumigation processing could be easily discriminated by chromatographic fingerprint and chemometrics. Conclusion: QAMS is a convenient and accurate approach to analyzing multi-component when reference substances are unavailable, simultaneously, chemometrics is an effective way to discriminate sun-dried and sulfur-fumigated Angelicae Sinensis Radix.