Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium

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Abstract
Pharmacognosy Magazine,2013,9,35,192-195.
Published:June 2013
Type:Original Article
Authors:
Author(s) affiliations:

Chun-Yan Guo1, Jin Wang2, Yong Hou2, Yong-Ming Zhao2, Li-Xia Shen2, Dan-Shen Zhang3
1Department of Graduate, HeBei Medical University, Shijiazhuang 050017, Hebei, China
2Department of Pharmacy, HeBei North University, Zhangjiakou, 075000, China
3Department of Graduate, HeBei Medical University, Shijiazhuang 050017, Hebei; HeBei University of Science and Technology, Shijiazhuang, 050018, China

Abstract:

Background: Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine. Materials and Methods: The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied through a single-factor experiment. An orthogonal array (L 9 (3 4 )) was then constructed to achieve the best extraction conditions. Results: Variance analysis revealed that ethanol concentration significantly affected the extraction yield. The optimal conditions were as follows: ethanol concentration, 50% (v/v); liquid to solid ratio, 15:1; duration of microwave irradiation, 240 s; and extraction temperature, 60°C. Conclusion: Under these optimal conditions, the total flavonoid yield was 18.34 ± 0.64 mg/g. The use of a microwave-assisted process dramatically reduced the time needed for extraction of flavonoids from I. helenium.

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