Standardization of spray-dried powder of Piper betle hot water extract

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Abstract
Pharmacognosy Magazine,2011,7,26,157-160.
Published:May 2011
Type:Short Communication
Authors:
Author(s) affiliations:

Liyanage Dona Ashanthi Menuka Arawwawala1, Horadugoda Gamage Sujatha Pushpakanthi Hewageegana2, Lakshmi Sriyani Rajapaksha Arambewela1, Hettiarachchige Sami Ariyawansa2
1Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 7, Sri Lanka
2Department of Nidana Chikitsa, Institute of Indigenous Medicine, University of Colombo, Rajagiriya, Sri Lanka

Abstract:

The leaves of Piper betle Linn. (Family: Piperaceae) possess several bioactivities and are used in the Traditional Medical systems of Sri Lanka. The present investigation was carried out to standardize the spray-dried powder of P. betle by (a) determination of physicochemical parameters, presence or absence of heavy metals, and microbial contamination; (b) screening for phytochemicals; and (c) development of High Pressure Liquid Chromatography (HPLC) fingerprint and densitogram. The percentages of moisture content, total ash, acid insoluble ash, water-soluble ash, and ethanol extractable matter of spray-dried powder of P. betle were 2.2-2.5, 6.8-7.0, 0.003-0.005, 4.1-4.3, and 15.8-16.2, respectively. The concentrations of all the tested heavy metals were below the WHO acceptable limits and bacterial species, such as Escherichia coliSalmonella spp, Staphylococcus aureus, and Pseudomonas aeroginosa were not present in the P. betle spray-dried powder. Phenolic compounds, tannins, flavonoids steroids, and alkaloids were found to be present in the spray-dried powder of P. betle and HPLC fingerprint and densitogram clearly demonstrated the proportional differences of these chemical constituents. In conclusion, the results obtained from this study can be used to standardize the spray-dried powder of P. betle.

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