Celery, chicory leaves, and barley grains are valuable in weight loss diets and regulate lipid metabolism. They may reduce risk of fatty liver. The present study aimed to investigate the effect of diet supplementation with celery, chicory, and barley powder on liver enzymes and blood lipids in rats fed with cholesterol-enriched diet. This study used four groups of rats fed with 3% cholesterol were supplemented diet to induce hypercholesterolemia and one group was fed on cholesterol-free basal diet. The dry powder of celery leaves, chicory leaves, and barley grains was separately added to the basal diet at 10% concentration or in combination of three plants at 15% for four weeks. Biochemical analyses of serum liver enzymes and blood lipids as well as histopathological examination of liver were performed. Feeding of diet supplemented with 10% of celery, 10% chicory, and 10% of barley lowered the elevated serum level of liver enzymes and blood lipids in rats. Feeding plant combination of celery, chicory, and barley at 15% concentration (5% from each) was more effective in decreasing the elevation of liver enzymes (aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase) and blood lipids. The histopathological lesions seen in the livers of hypercholesterolemic rats were ameliorated by feeding this plant mixture. This study recommends that dietary intake of plant mixture of celery; chicory, and barley at 15% (5% of each) concentration can be beneficial to patients suffering from hypercholesterolemia and liver diseases.