The standardized extract of Limonium tetragonum Alleviates chronic alcoholic liver injury in C57BL/6J Mice

Na-Hyun Kim; Jeong-Doo Heo; Jung-Rae Rho; Min Hye Yang; Eun Ju Jeong

Articles

Abstract

Pharmacognosy Magazine,2018,14,53,58-63.

DOI:10.4103/pm.pm_44_17

Published:February 2018

Type:Original Article

Authors:

Author(s) affiliations:

Na-Hyun Kim¹, Jeong-Doo Heo¹, Jung-Rae Rho², Min Hye Yang³, Eun Ju Jeong⁴
¹ Gyeongnam Department of Environment and Toxicology, Korea Institute of Toxicology, Gyeongnam 52834, Republic of Korea
² Department of Oceanography, Kunsan National University, Jeonbuk 54150, Republic of Korea
³ College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea
⁴ Department of Agronomy and Medicinal Plant Resources, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea

Abstract:

Background: In traditional folk medicine, Limonium tetragonum is used in the treatment of uterine hemorrhage, tinnitus, and oligomenorrhea. Objective: This study aimed to identify the therapeutic effect of L. tetragonum EtOAc extract (EALT) on liver of mice with chronic alcohol poisoning. Materials and Methods: C57BL/6J mice were administered 100 mg/kg of EALT with a single binge ethanol/Lieber-DeCarli liquid diet for 8 weeks. Results: The chronic-binge ethanol diet induced a significant increase in liver marker enzyme activities. Coadministration of EALT reversed the elevation of serum total cholesterol and triglyceride as well as aspartate aminotransferase and alanine aminotransferase due to chronic alcohol consumption. Histologic findings including markedly attenuated fat accumulation in hepatocytes were observed in EALT-treated mice. EALT supplementation prevented alcoholic liver injury through attenuation of inflammatory mediators such as toll-like receptor-4, cytochrome P4502E1, and cyclooxygenase-2, and inflammatory cytokine interleukin-6. Conclusion: Results provided direct experimental evidence for the hepatoprotective effect of EALT in the NIAAA mouse model. Therapeutic attempts with the L. tetragonum extract might be useful in the management of alcoholic liver disease.