Fracture healing in SD rats&#039; bioactivities of cucumber seed polysaccharides

Zhihong Zhang; Yuanning Zeng; Donghui Peng; Na Xing; Haodong Bai; Yuxin Li; Na Cui; Huilin Su; Tingting Qiao; Congjing Shi; Haixue Kuang; Qiuhong Wang

Articles

Abstract

Pharmacognosy Magazine,2022,18,79,738-745.

DOI:10.4103/pm.pm_14_22

Published:September 2022

Type:Original Article

Authors:

Author(s) affiliations:

Zhihong Zhang¹, Yuanning Zeng², Donghui Peng¹, Na Xing³, Haodong Bai¹, Yuxin Li¹, Na Cui¹, Huilin Su², Tingting Qiao², Congjing Shi², Haixue Kuang¹, Qiuhong Wang⁴
¹Department of Chinese Medicine Processing, Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China
²Department of Chinese Medicine Processing, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China
³Zhongshan Institude for Drug Discovery, Zhongke Zhongshan Institute of Drug Innovation, Zhongshan, China
⁴Department of Chinese Medicine Processing, Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin; Department of Chinese Medicine Processing, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China

Abstract:

Aim/Background: We investigated the composition and structure of intracellular polysaccharides in cucumber seeds (CSs) and their effect on Sprague-Dawley (SD) rats and potential activity for fracture healing. Materials and Methods: Polysaccharides are its main water-soluble active ingredient. The SD rat tibia fracture model was established by surgical method, and the healing effect of cucumber seed polysaccharides (CSPs) on SD rat tibia fracture was preliminarily explored. Through histopathological observation, determination of rat serum indicators alkaline phosphatase (AKP), acid phosphatase (ACP), BGP, X-ray imaging observation, and micro-Computed Tomography (CT) imaging observation at different periods. Results and Conclusion: The results showed that CSP enhanced the activity of osteoblasts, increased the metabolic rate of osteoclasts, increased the secretion of AKP, BGP, and ACP, shortened the fracture healing time of SD rats and effectively promoted the healing of tibial fractures in SD rats.

Keywords:Cucumber seeds, extraction of polysaccharides, Fracture healing, polysaccharides, SD rats