Objectives: Physalis peruviana L. is a medicinal herb and its consumption increases annually in The Middle East, also the scientific research on it increases due to its valuable nutrient. Materials and Methods: Methanolic extracts of P. peruviana L. seeds and juice were screened for their anticancer and antioxidant. Gas chromatography-mass spectroscopy profiling was performed for all extracts. . Results: The identification of seeds and juice methanolic extract showed the main sex compounds; ethanol, 2,2'-oxybis-, caffeic acid in both of the extracts. Octadecadienoate ethyl and octadecenoic acid have been found in seed extract, and octadecadienoic acid and ferulic acid were in juice extract. Seeds extract has phenolic and flavonoid content as 53.58 and 45.56, respectively, comparing to juice extract (26.58 and 7.30, respectively). The antioxidant activities of seeds extract using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant potential (FRAP) (28.73 at 50 μg/ml and 1164.10, respectively) comparing to juice extract values (4.06 at μg/ml and 848.43, respectively). Conclusion: The conspicuous optimistic result is that seeds extract showed cancer inhibition against human HepG2 and A549 (81.45 and 85.34, respectively) comparing to juice extract (44.06 and 32.06, respectively). Therefore, the demand to increase the usage of Physalis or golden berry in people's diet is a demand to face the environmental oxidative stress.