Background: Plant-derived products have a vital biological role against various diseases. The present study was carried out to document and investigate the existing ethnomedicinal knowledge on local flora of Kapurthala district, Punjab, India. Materials and Methods: The ethnomedicinal investigation was carried out from March 2015 to October 2017. Key informants, rural as well as urban, i.e., herbal doctors, homemakers, farmers, elders, etc., were selected using snowball sampling method. Information on ethnomedicinal importance of plants of this region and methods of preparation and administration was gathered by questionnaire-based personal interviews, group discussions, informal meetings, and field observations with local people. Results: Fifty plant species belonging to thirty families were informed to be used for medicinal purposes. Fruits and leaves were the most used plant parts. The most commonly used mode of administration was raw form or juice of plant parts. Most of the information was recorded for the treatment of gastrointestinal problems. Conclusions: This study reveals the interesting use of plants by the local people of Kapurthala district, which shows that use of medicinal plants still exists. However, because of modernization, traditional knowledge is losing at high rate; there is a need to conserve our ancestral knowledge. Further, medicinal plants should be examined for pharmacological and phytochemical studies for novel drug discovery.