DNA barcoding markers to identify intraspecies genetic variations in three ecotypes of Abrus Precatorius L.

Srinivasan Marimuthu; GP Pratap; Goli Penchala Prasad; Shyam Baboo Prasad; Anupam Kumar Mangal; N Srikanth

Articles

Abstract

Pharmacognosy Magazine,2020,16,05,540-545.

DOI:10.4103/pm.pm_23_20

Published:November 2020

Type:Original Article

Authors:

Author(s) affiliations:

Srinivasan Marimuthu¹, GP Pratap², Goli Penchala Prasad³, Shyam Baboo Prasad⁴, Anupam Kumar Mangal⁵, N Srikanth⁶
¹ Research Officer (Biochemistry), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India
² Survey of Medicinal Plants Unit, Central Research Institute for Unani Medicine, Hyderabad, Telangana, India
³ Assistant Director In-charge, Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India
⁴ Research Officer (Pharmacognosy), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India
⁵ Assistant Director (Pharmacognosy), Central Council for Research in Ayurvedic Sciences, New Delhi, India
⁶ Deputy Director General, Central Council for Research in Ayurvedic Sciences, New Delhi, India

Abstract:

Background: Abrus precatorius is also called as Gunja in Ayurveda. It is a commonly grown plant belonging to the family of Fabaceae. It is characterized under the Upavisha (semi-poisonous drugs) and used widely in various Ayurvedic formulations with great beneficial significance. It is used in the treatment of various diseases such as alopecia, edema, helminthiasis, skin diseases, itching, and urinary disorders after being passed through specific purification procedures. Objectives: The present study aims to compare three different varieties of A. precatorius which includes the species of white, black, and red which are used to study DNA barcoding marker and phylogenetic analysis. Materials and Methods: Whole genomic isolated DNA from three varieties (white, black, and red) of A. precatorius leaves and subjected to analysis of the polymerase chain reaction of rbcL, maturase K, and internal transcribed spacer 4,5 using 0.8% agarose gel electrophoresis. Results: The DNA barcoding markers and next-generation sequencing can identify the intraspecies genetic variations among these closely related plant varieties of A. precatorius of white, black, and red. Conclusion: The intraspecies genetic variations among these three varieties of A. precatorius white, black, and red are closely related with A. precatorius isolate TMP 144 Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene 99.81%.