Phytochemical evaluation and cytotoxic potential of chloroform soluble fraction of methanol extract of Thespesia populnea in human breast cancer cell lines

Anu Gopalakrishnan; Bibu John Kariyil; Reni John; PT A. Usha

Articles

Abstract

Pharmacognosy Magazine ,2019,15,62,150-154.

DOI:10.4103/pm.pm_329_18

Published:April 2019

Type:Original Article

Authors:

Author(s) affiliations:

Anu Gopalakrishnan, Bibu John Kariyil, Reni John, PT A. Usha
Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Mannuthy, Thrissur, Kerala, India

Abstract:

Background: Thespesia populnea (Poovarasu), belonging to family Malvaceae, is a tropical evergreen tree found abundantly in the coastal regions of India. It is traditionally known for its hepatoprotective, antitumor, antioxidant, and wound healing activities. Objective: The present study was undertaken to find out different phytochemical principles of chloroform-soluble fraction of T. populnea (CSFTP) using gas chromatography mass spectrometry-mass spectrometry (GCMS-MS) and Fourier-transform infrared (FTIR) spectroscopy and to analyze in vitro cytotoxic potential of the fraction in MDA-MB-231 and MCF-7 human breast carcinoma cell lines. Materials and Methods: Shade-dried bark of T. populnea was extracted using methanol and fractionated to obtain CSF. The fraction was subjected to GCMS, FTIR, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Results: GCMS-MS and FTIR analysis showed the presence of various steroids and fatty acid analogs. Cell growth inhibition was noted for CSFTP in MDA-MB-231 and MCF-7 cell lines. Conclusion: CSFTP revealed the presence of various active principles which could be attributed for its cytotoxic potential.