Chemical profiles by thin-layer chromatography and high-performance liquid chromatography of plant species from Northeast Brazil

Julia Aparecida Lourenco de Souza; Wliana Alves Viturino da Silva; Isabelle Cristinne Ferraz Bezerra; Magda Rhayanny Assunção Ferreira; Luiz Alberto Lira Soares

Articles

Abstract

Pharmacognosy Magazine,2018,14,56,437-443.

DOI:10.4103/pm.pm_225_17

Published:August 2018

Type:Original Article

Authors:

Author(s) affiliations:

Julia Aparecida Lourenco de Souza¹, Wliana Alves Viturino da Silva², Isabelle Cristinne Ferraz Bezerra³, Magda Rhayanny Assunção Ferreira³, Luiz Alberto Lira Soares⁴
¹ Postgraduate Program in Pharmaceutical Sciences, Federal University of Pernambuco; Department of Pharmaceutical Sciences, Laboratory of Pharmacognosy, Federal University of Pernambuco, Recife, PE, Brazil
² Department of Pharmaceutical Sciences, Laboratory of Pharmacognosy, Federal University of Pernambuco, Recife, PE, Brazil
³ Department of Pharmaceutical Sciences, Laboratory of Pharmacognosy, Federal University of Pernambuco; Postgraduate Program in Therapeutic Innovation, Federal University of Pernambuco, Recife, PE, Brazil
⁴ Postgraduate Program in Pharmaceutical Sciences, Federal University of Pernambuco; Department of Pharmaceutical Sciences, Laboratory of Pharmacognosy, Federal University of Pernambuco; Postgraduate Program in Therapeutic Innovation, Federal University of Pernambuco, Recife, PE, Brazil

Abstract:

Background: Fingerprint analysis plays a key role in quality control of herbal medicines due to its technical capacity to represent the chemical diversity of these complex matrices. Several traditional Brazilian species showed very little data in their chemical profiles. Objective: Thus, the purpose of this study was to evaluate the chemical profiles of Brazilian herbal species by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Materials and Methods: The herbal materials of 7 species (Anacardium occidentale, Annona muricata, Guazuma ulmifolia, Phyllanthus niruri, Psidium guajava, Punica granatum, and Spondias mombin) were collected from three different locations in Northeast Brazil, botanically authenticated and their chemical profile analyzed by TLC (cinnamics, flavonoids, and tannins) and by HPLC (polyphenols). Results: The chromatographic data showed the similarities between chemical profiles of the sample fingerprints, confirming the presence of several classes of secondary metabolites as well as the identification of different chemical standards (catechin, chlorogenic acid, caffeic acid, ellagic acid, gallic acid, quercetin, or rutin). Conclusion: The chromatographic profiling of the herbal drugs by TLC and HPLC were successfully characterized and allowed for the identification of promising chemical markers, improving the state of art in the quality control of the herbal species investigated in this study.