Flavonoid profiles of three Bupleurum species and in vitrohepatoprotective activity of Bupleurum flavum Forsk.

Background: Bupleurum L. (Aspiaceae) species are used as herbal remedy in Chinese traditional medicine.
Objective: The aim was to investigate the flavonoids in three annual European Bupleurum species, including B. baldense, B. affine and B. flavum, and to test their antioxidant and possible hepatoprotective effects.
Materials and Methods: Flavonoids from the methanol-aqueous extracts were quantified by solid-phase extraction-high-performance liquid chromatography. Bupleurum extracts (1-220 mg/ml) were tested for their antioxidant activity in DPPH and ABTS assays, as well as on isolated liver rat microsomes. In vitro hepatoprotective activity of B. flavum flavonoid (BFF) mixture and rutin, and narcissin, isolated from the same mixture, were evaluated on carbon tetrachloride (CCl ₄ ) and tert-butyl hydroperoxide (t-BuOOH) toxicity models in isolated rat hepatocytes.
Results: Narcissin was the dominant flavonol glycoside in B. flavum being present at 24.21 ± 0.19 mg/g, whilst the highest content of rutin (28.63 ± 1.57 mg/g) was found in B. baldense. B. flavum possessed the strongest DPPH (IC ₅₀ 22.12 μg/ml) and ABTS (IC ₅₀ 118.15 μg/ml) activity. At a concentration 1 mg/ml of BFF (rutin 197.58 mg/g, narcissin 75.74 mg/g), a stronger antioxidant effect in microsomes was evidenced in comparison with silymarin, rutin and narcissin. The hepatoprotective effect of BFF significantly reduced the elevated levels of lactate dehydrogenase and malondialdehyde, and ameliorated glutathione, being most active in t-BuOOH-induced injury model when compared with CCl ₄ toxicity (P < 0.001).
Conclusion: In BFF, synergism of rutin and narcissin could be responsible for stronger protection against mitochondrial induced oxidative stress.