Exploring the beneficial role of ononin in preventing ovariectomy-induced osteoporosis in rats via Osx and Runx2 pathway

Tonghao Wang; Lijun Tian; Lilong Du; Guowang Li; Yongjin Li; BaoShan Xu

Articles

Abstract

Pharmacognosy Magazine,2022,18,80, 1055-1065.

DOI:10.4103/pm.pm_282_21

Published:November 2022

Type:Original Article

Authors:

Author(s) affiliations:

Tonghao Wang¹, Lijun Tian², Lilong Du³, Guowang Li⁴, Yongjin Li⁴, BaoShan Xu⁵
¹Clinical College of Orthopedics, Tianjin Medical University; Department of Orthopedic, The Third Central Hospital of Tianjin, Tianjin, China
²Clinical College of Orthopedics, Tianjin Medical University, Tianjin; Department of Orthopedic, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology Baotou, Inner Mongolia, China
³Department of Minimally Invasive Spine Surgery, Tianjin Hospital, Jiefang South Road 406, Hexi District, Tianjin, China
⁴Graduate School, Tianjin Medical University, Tianjin, China
⁵Clinical College of Orthopedics, Tianjin Medical University; Department of Minimally Invasive Spine Surgery, Tianjin Hospital, Jiefang South Road 406, Hexi District, Tianjin, China

Abstract:

Background: Osteoporosis is a multifactorial bone disease that progresses without notice until attaining a chronic stage. It causes socio-economic burden, higher mortality rate, and increased bone fractures. Ononin, an isoflavone present in numerous herbal Chinese medicinal formulations such as Astragali radix and red clover, has been reported to have potent therapeutic properties. Objectives: In this study, we aimed to evaluate the efficacy of ononin against ovariectomy(OVX)-induced osteoporosis in female rats. Materials and Methods: Ovaries were removed surgically to develop the animal model of OVX. Animals (n = 4) were grouped into control, OVX induced, OVX rats treated with ononin (10 mg/kg body weight), and OVX rats treated with 20 mg/kg body weight of ononin. Results: OVX rats treated with ononin showed improved body weight, uterus index, inflammatory and bone markers, serum lipid profile, and calcium level. Ononin downregulated the expression of estrogen (E2), acid phosphatase, and bone gamma-carboxyglutamate (Gla) protein in OVX rats. It also restored trabecular area and thickness, which was evidenced by histomorphometrical and histopathological examination. Ononin attenuated the expression of alkaline phosphatase, osterix, and Runx2 in OVX rats. Conclusion: In summary, our results suggest that ononin could be used as an ideal therapeutic agent to prevent and treat bone-associated disorders.