Determination of 5-hydroxymethyl-2-furaldehyde of crude and processed fructus corni in freely moving rats using in vivo microdialysis sampling and liquid chromatography

Enhong Ouyang; Chengrong Zhang; Xiaomeng Li

Articles

Abstract

Pharmacognosy Magazine,2011,7,28,271-276.

DOI:10.4103/0973-1296.90394

Published:November 2011

Type:Original Article

Authors:

Author(s) affiliations:

Enhong Ouyang¹, Chengrong Zhang², Xiaomeng Li²
¹Department of Pharmaceutics, Shaoyang Medical College, Shangyang 422000, China
²Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 310053, China

Abstract:

Background: Fructus Corni is derived from the dry ripe sarcocarp of Cornus officinalis Sieb. et Zucc. 5-hydroxymethyl-2-furaldehyde (5-HMF) is an important active composition of the Fructus Corni. However, there have been no reports on the concentration of 5-HMF in freely moving rats using microdialysis coupled with HPLC. Materials and Methods: The concentration of 5-HMF in free-moving rats after intra-gastric (i.g.) administration of the water extract of Fructus Corni and JZP was analyzed by microdialysis coupled with high-performance liquid chromatographic (HPLC). Results: Results demonstrated that the concentration of 5-HMF in microdialysate was 1.4951 μg/l, but higher in rat microdialysate after i.g. administration of the aqueous extract of JZP (5.2662 μg/l). Conclusion: This method is proved to be rapid, accurate and simple. Real-time in vivo monitoring the concentration of 5-HMF provides the theoretical basis for further explaining the processing mechanism of Fructus Corni.