Ellagic acid ameliorates lung injury after intestinal ischemia-reperfusion

Abdullah Böyük; Akın Önder; Murat Kapan; Metehan Gümüş; Uğur Fırat; Mustafa Kemal Başaralı; Harun Alp

Articles

Abstract

Pharmacogn Mag,2011,7,27,224-228.

DOI:10.4103/0973-1296.84236

Published:August 2011

Type:Original Article

Authors:

Author(s) affiliations:

Abdullah Böyük, Akın Önder, Murat Kapan, Metehan Gümüş, Uğur Fırat¹, Mustafa Kemal Başaralı², Harun Alp³

Departments of General Surgery,¹ Pathology and² Biochemistry, Medical School, Dicle University, Diyarbakır,³ Department of Pharmacology and Toxicology, Veterinary School, Dicle University, Diyarbakır, Turkey

Abstract:

Background: The aim of this study was to investigate the possible protective role of antioxidant treatment with ellagic acid (EA) on lung injury after intestinal ischemia-reperfusion (I/R) injury using biochemical and histopatological approaches. Materials and Methods: Forty rats were divided into four groups as control, control + EA, I/R, and I/R + EA. The control and control + EA groups were also anesthetized and subjected to laparotomy, but without clamp application. The control + EA and I/R + EA groups were given EA (85 mg/kg) orally prior to experiment. The I/R and I/R + EA groups underwent 30 minutes of intestinal ischemia and 1 hour of reperfusion. In all groups, serum total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were determined. TAC, total oxidative status (TOS), and oxidative stress index (OSI) in lung tissue were measured. Lung tissue histopathology was also evaluated by light microscopy. Results: TAC levels were higher in control, EA, and I/R + EA groups while TOS, OSI, and MDA levels were lower in these groups compared with I/R group. Serum MDA levels were significantly higher in I/R + EA group than that of control group. Lung tissue TAC levels were lower in I/R + EA group while OSI values were higher in that groups compared with EA group. Histological tissue damage was milder in the EA treatment group than in the I/R group. Conclusion: These results suggest that EA treatment protected the rats lung tissue against intestinal I/R injury.