Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography

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Abstract
Pharmacogn Mag,2010,6,22,98-101.
DOI:10.4103/0973-1296.62893
Published:April 2010
Type:Original Article
Authors:
Author(s) affiliations:

Xiaoying Zhou1, Xiao-Ming Chen2, Liang Ge3, Hai-Yan Gong3, Shuge Tian3, Dongqing An3
1Xinjiang Key Laboratory of Famous Prescription and Science of Formulas; and College of Pharmacy, XinJiang Medical University, Urumqi - 830 011, China
2Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Scotland-G1 1QE, United Kingdom
3Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi - 830 011, China

Abstract:

A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C 18 4.6 mmΧ50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.

Keywords:Paeonia sinjiang K. Y. Pan, paeoniflorin, rapid resolution liquid chromatography

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Keywords
rapid resolution liquid chromatography
Paeonia sinjiang K. Y. Pan
paeoniflorin