Catechin isolated from Garcinia celebica leaves inhibit Plasmodium falciparum growth through the induction of oxidative stress

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Abstract
Pharmacognosy Magazine,2017,13,50s,s301-s305.
Published:July 2017
Type:Original Article
Authors:
Author(s) affiliations:

Rizky Abdulah1, Eka W Suradji2, Anas Subarnas3, Unang Supratman4, Milyadi Sugijanto3, Ajeng Diantini3, Keri Lestari3, Melisa I Barliana5, Shinichiro Kawazu6, Hiroshi Koyama7
1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia; Department of Public Health, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan
2Department of Public Health, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan; Teluk Bintuni Hospital, Teluk Bintuni Regency, West Papua 98364, Indonesia
3Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia
4Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jatinangor 45363, Indonesia
5Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor 45363, Indonesia
6National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
7Department of Public Health, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan

Abstract:

Background: Resistance of antimalarial drugs to Plasmodium falciparum has become a major concern in malaria eradication. Although it is also affected by several socioeconomic factors, a new antiplasmodial agent is needed for a global malaria control program. Objective: In this study, we attempted to uncover the antiplasmodial properties of Garcinia celebica, an Indonesian medicinal plant, along with the responsible compound and its possible mechanism. Materials and Methods: The G. celebica leaves were ethanol extracted and fractionated based on their polarity using n-hexane, ethyl acetate, and water. The antiplasmodial activity was tested in vitro against chloroquine-resistant P. falciparum at 100 μg/ml for 72 h. The active compound of the most active ethyl acetate fraction was subsequently isolated using column chromatography and identified by nuclear magnetic resonance. Results: The IC50of (+)-catechin, the characterized compound, against P. falciparum was 198 μM in 24 h and experiment. The isolated catechin inhibited P. falciparum growth in both trophozoite and schizont stages. An additional experiment also suggests that the antiplasmodial property of catechin occurs through the induction of the oxidative stress to P. falciparumConclusion: This result shows that the potential of catechin and its antimalarial properties should be explored further.

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