Biological activities and cytotoxicity of Eperua oleifera Ducke oil-resin

Fernanda Torlania Alves Gomes; Ana Paula de Araújo Boleti; Lidiam M Leandro; Diego Squinello; Diego Squinello; Marne C Vasconcelos; Paul Cos; Valdir F Veiga; Emerson Silva Lima

Articles

Abstract

Pharmacognosy Magazine ,2017,13,52,542-552.

DOI:10.4103/pm.pm_552_16

Published:November 2017

Type:Original Article

Authors:

Author(s) affiliations:

Fernanda Torlania Alves Gomes¹, Ana Paula de Araújo Boleti¹, Lidiam M Leandro², Diego Squinello², Ellen S. P. Aranha¹, Marne C Vasconcelos¹, Paul Cos³, Valdir F Veiga², Emerson Silva Lima¹
¹Biological Activity Laboratory, Faculty of Pharmaceutical Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil
²Chemistry Department, Exact Sciences Institute, Federal University of Amazonas, Manaus, Amazonas, Brazil
³Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, B2610 Antwerp, Belgium

Abstract:

The oil-resin of|This study aimed to investigate the effects of the acid fraction of|Acid fraction of|The predominant acidic diterpenes in the AFEOR were copalic and hardwickiic acids. AFEOR caused morphology alteration and decrease of proliferation at concentrations higher than 5 μg/mL. It also caused significant collagen proliferation in fibroblasts. It showed cytotoxicity against tumoral and nontumoral cell lines, with IC|For the first time, biological activities from oil-resin|Analysis of crude oil-resin and fractionation of diterpenic fraction was performance using selective ion-exchange column chromatographyCytotoxicity analysis and morphology were performed with different cell linesCollagen production in human fibroblasts, inhibition of metalloproteinases were demonstrated by zymography and Western blotting.

Keywords:collagenase, Eperua, metalloproteinases, tumoral cell