Chemical composition and biological potential of a chloroform fraction from the leaves of marine plant Syringodium filiforme Kützing

Kethia Gonzalez Garcia; David Marrero Delange; Yasnay Hernández Rivera; Yulexi Acosta Suárez; Richard Gutiérrez Cuesta; Mario Riera-Romo; Olga Echemendia; Lívia Macedo Dutra; Jackson Roberto Guedes da Silva Almeida; Dayana Pérez-martínez; Laurent Picot; Idania Rodeiro Guerra

Articles

Abstract

Pharmacognosy Magazine,2020,16,72,750-756.

DOI:10.4103/pm.pm_24_20

Published:February 2021

Type:Original Article

Authors:

Author(s) affiliations:

Kethia Gonzalez Garcia¹, David Marrero Delange¹, Yasnay Hernández Rivera¹, Yulexi Acosta Suárez¹, Richard Gutiérrez Cuesta¹, Mario Riera-Romo², Olga Echemendia³, Lívia Macedo Dutra⁴, Jackson Roberto Guedes Da Silva Almeida⁴, Dayana Pérez-martínez⁵, Laurent Picot⁶, Idania Rodeiro Guerra²
¹ Department of Chemistry, Institute of Marine Science, Revolución, Havana, Cuba
² Department of Pharmacology, Institute of Marine Science, Havana, Cuba
³ Carlos J. Finlay Research Institute, Havana, Cuba
⁴ Department of Pharmacy, Federal University of Vale Do São Francisco, Petrolina, Pernambuco, Brazil
⁵ Center For Molecular Immunology, Directorate of Tumor Immunology, Havana, Cuba
⁶ Faculty of Science and Technology, UMRI CNRS Lienss, La Rochelle University, La Rochelle, France

Abstract:

Background: Seagrasses are an important component of Nearshore marine ecosystems and a rich source of secondary metabolites with important pharmacological properties. Materials and Methods: In this work, crude hydroethanolic extract and chloroform fraction (SfCHCl3) from marine plant Syringodium filiforme were evaluated for antimicrobial and cytotoxic potentials. In addition, the chemical composition of chloroform fraction was determined by gas chromatography-mass spectrometry (GC-MS) analysis. Results: GC-MS analysis allowed the identification of 68 compounds in the SfCHCl3, where palmitic acid (39.18%) was the main component. The evaluation of antibacterial activity of SfCHCl3 showed good activity against strains of Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 9027), and Salmonella typhi (ATCC 9992v), and it was less active against Escherichia coli (ATCC 10576) and Candida albicans (ATCC 10231), while the crude extract showed low antibacterial activity. Furthermore, an important cytotoxic effect of SfCHCl3 in the A549 human lung carcinoma cell line was evidenced, which were similar to the cytotoxicity of cisplatin in the same cell line. Conclusion: The results suggest that compounds may play an important role in the antimicrobial and cytotoxic effects observed for this species. In addition, this research contributes to the chemotaxonomic characterization of S. filiforme and validates this species as a potential source of natural antimicrobial and cytotoxic molecules.