Extraction, isolation and identification of kaempferol 3,7 – Diglucoside in the leaf extracts of Evolvulus alsinoides (Linn.) and its inhibition potency against α-amylase, α-glucosidase, Acetylcholinesterase and amyloid aggregation

Background: Evolvulus alsinoides (Linn.) is an eminent kaphahara (balancing kapha ) plant in Ayurvedic medicine commonly used as brain tonic. Objectives: To report the enzyme inhibitory potency of the plant extract and to isolate the compound of significant impact. Materials and Methods: The plant extracts were obtained by serial exhaustive extraction and successive chromatographic separation. Antioxidant and enzyme inhibition assays were carried out to extract the required isolate. Results: The current research identifies for the first time, the presence of Kaempferol 3,7 diglycoside in the leaf extracts by Spectrophotometry, Chromatography,[1]H and[13]C Nuclear Magnetic Resonance, carboxylic and hydroxylic hydrolysis. The isolated and characterized compound possesses enzyme inhibition property towards α-amylase, α-glucosidase and Acetylcholinesterase . Conclusion: The compound protects differentiating neuronal cells, SH-SY5Y from Amyloid β Peptide-induced Injury. Thus, the newly identified compound could serve as a plant-based bioactive in the management of Alzheimer's.