Neuroprotective effects of 4,5-dimethoxypyrocatechol isolated from Cynanchum paniculatum on HT22 cells

Jin Bae Weon; Bohyoung Lee; Bo-Ra Yun; Jiwoo Lee; Choong Je Ma

Articles

Abstract

Pharmacognosy Magazine,2014,10,38,161-164.

DOI:10.4103/0973-1296.131028

Published:April 2014

Type:Original Article

Authors:

Author(s) affiliations:

Jin Bae Weon¹, Bohyoung Lee¹, Bo-Ra Yun¹, Jiwoo Lee¹, Choong Je Ma²
¹ Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Korea
² Department of Medical Biomaterials Engineering, College of Biomedical Science; Research Institute of Biotechnology, Kangwon National University, Chuncheon, Korea

Abstract:

Background: Cynanchum paniculatum, belongs to the family Asclepiadaceae and is used to treat various diseases, such as invigorate blood, alleviate edema and to relieve pain and toxicity for a long time. Materials and Methods: 4,5-Dimethoxypyrocatechol was isolated from the 80% methanol extract of C. paniculatum and its neuroprotective effect was evaluated by MTT assay. Results: 4,5-Dimethoxypyrocatechol had neuroprotective effect on the glutamate-induced cellular oxidative death in HT22 cells. Conclusion: Furthermore, we found that reactive oxygen species (ROS) accumulation and calcium concentration by oxidative stress were reduced by 4,5-dimethoxypyrocatechol in HT22 cells.