Quality analysis of chlorogenic acid and hyperoside in Crataegi fructus

Jin Bae Weon; Youn Sik Jung; Choong Je Ma

Articles

Abstract

Pharmacognosy Magazine,2016,12,46,98-103.

DOI:10.4103/0973-1296.177904

Published:March 2016

Type:Original Article

Authors:

Author(s) affiliations:

Jin Bae Weon¹, Youn Sik Jung¹, Choong Je Ma²
¹ Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea
² Department of Medical Biomaterials Engineering, College of Biomedical Science; Department of Biomaterials Engineering, Kangwon National University, Chuncheon 200-701, Korea

Abstract:

Background: Crataegi fructus is a herbal medicine for strong stomach, sterilization, and alcohol detoxification. Chlorogenic acid and hyperoside are the major compounds in Crataegi fructus. Objective: In this study, we established novel high-performance liquid chromatography (HPLC)-diode array detection analysis method of chlorogenic acid and hyperoside for quality control of Crataegi fructus. Materials and Methods: HPLC analysis was achieved on a reverse-phase C₁₈ column (5 μm, 4.6 mm × 250 mm) using water and acetonitrile as mobile phase with gradient system. The method was validated for linearity, precision, and accuracy. About 31 batches of Crataegi fructus samples collected from Korea and China were analyzed by using HPLC fingerprint of developed HPLC method. Then, the contents of chlorogenic acid and hyperoside were compared for quality evaluation of Crataegi fructus. Results: The results have shown that the average contents (w/w %) of chlorogenic acid and hyperoside in Crataegi fructus collected from Korea were 0.0438% and 0.0416%, respectively, and the average contents (w/w %) of 0.0399% and 0.0325%, respectively. Conclusion: In conclusion, established HPLC analysis method was stable and could provide efficient quality evaluation for monitoring of commercial Crataegi fructus.