Background: Dolichandrone serrulata flower has been known for its antioxidation and anti-inflammation. Its ability to induce cell death and reduce migration on cancer cell lines has been previously demonstrated. Aim: The present study aimed to investigate the molecular mechanisms involved in the antimigration effect of D. serrulata flower ethanolic extracts of HeLa cell line. Materials and Methods: HeLa cell line viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antimigration of the extract was investigated using a wound healing assay. Its possible molecular mechanism for the migration of HeLa cell line was investigated using a protein array assay. Results: D. serrulata extract caused cell death with CC50 of 232.10 ± 17.16 μg/mL. The result from the wound healing assay demonstrated that D. serrulata extracts at concentrations of 62.5, 125, and 250 μg/mL caused the reduction of the narrowing in the wound site, corresponding to the progress over time. To eradicate the possibility of the cytotoxic effect of the herb, D. serrulata at a concentration of 125 μg/mL was further observed for protein expression using a protein array assay. The result showed the reduction of galactin-3, vimentin, and fibroblast growth factor-2 expression compared with the control group. Conclusion: Antimigratory effect of D. serrulata flower ethanolic extracts might be attributable to the reduction of vimentin and fibroblast growth factor-2 gene expression, but not galactin-3-induced apoptosis. Further study is needed to confirm and investigate a more in-depth pathway on its antimigration effect in the cervical cancer cell line.