The effects of Elateriospermum tapos against obese maternal rat in mitigating obesity development among their adult female offspring

Hasnah Bahari; Azrina Zainal Abidin; Santhra Segaran Balan; Kokila Vani Perumal; Nurain Syahirah Rosli; Amalia Hazirah Ahamad Lotafi; Sharmmila Danabala; Malarmugila Manimaran; Nurul Husna Shafie; Maizaton Atmadini Abdullah; Azmiza Syawani Jasni

Articles

Abstract

Pharmacognosy Magazine,2020,16,72,706-712.

DOI:10.4103/pm.pm_142_20

Published:February 2021

Type:Original Article

Authors:

Author(s) affiliations:

Hasnah Bahari¹, Azrina Zainal Abidin², Santhra Segaran Balan², Kokila Vani Perumal¹, Nurain Syahirah Rosli³, Amalia Hazirah Ahamad Lotafi³, Sharmmila Danabala³, Malarmugila Manimaran³, Nurul Husna Shafie⁴, Maizaton Atmadini Abdullah⁵, Azmiza Syawani Jasni⁶
¹ Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia
² Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia; Department of Diagnostic and Allied Health Science, Faculty of Health and Life Sciences, Management and Science University, Malaysia
³ Department of Diagnostic and Allied Health Science, Faculty of Health and Life Sciences, Management and Science University, Malaysia
⁴ Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia
⁵ Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia
⁶ Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia

Abstract:

Background: Maternal obesity is a metabolic disorder described by chronic inflammation and dyslipidemia and influences non-communicable diseases in offspring. Elateriospermum tapos is a plant containing high alpha-linolenic acid with antiobesity effect. Objectives: This study was aim to investigate the effect of E. tapos supplementation in obese rat prior pregnancy on the female offspring's body weight, liver and kidney profile, and histological changes of heart, liver, kidney, and retroperitoneal white adipose tissue (RpWAT). Materials and Methods: Thirty female Sprague Dawley rats were used in this study. Six rats were assigned to dams normal diet group fed with standard chow diet. The remaining rats were fed with high-fat and cafeteria diet (HFCD) to generate obesity for 5 weeks. The obese rats were further divided into 4 groups; group1; Dams Negative Control (DNC, normal saline only), group 2; Dams Positive Control (DPC, Orlistat drug 200mg/kg), Dams Treatment 1 (DTX1, E. tapos seed 200 mg/kg) and Dams Treatment 2 (DTX2, E. tapos shell 200mg/kg) extracts for 6 weeks daily prior to mating. The weaning offspring were then designated into 6 groups according to their dam's group (n = 6/group). The offspring were culled at 12th week of age in order to have their blood sample and organs. Results: Adult offspring from DTX2 group showed a decrease in weight gain, alanine aminotransferase, and creatinine level compared to adult offspring from DNC. Histological examination showed that liver and RpWAT of offspring from DTX1 and DTX2 groups were comparable with normal diet group, indicating that the extracts were capable in reversing the high fat diet effect to the organ. Conclusion: E. tapos shell supplementation was effective in reducing the development of obesity with no harmful effect among adult female rat offspring.