Antihyperglycemic activity of the leaves from annona diversifolia Safford. and farnesol on normal and alloxan-induced diabetic mice

Fernando Calzada; Miguel Valdés; Normand García-Hernández; Claudia Velázquez; Elizabeth Barbosa; Celia Bustos-Brito; Leovigildo Quijano; Emmanuel Pina-Jiménez; Jesica Elena Mendieta-Wejebe

Articles

Abstract

Pharmacognosy Magazine ,2019,15,62,5-11.

DOI:10.4103/pm.pm_582_18

Type:Original Article

Authors:

Author(s) affiliations:

Fernando Calzada¹, Miguel Valdes², Normand Garcia-Hernandez³, Claudia Velázquez⁴, Elizabeth Barbosa⁵, Celia Bustos-Brito⁶, Leovigildo Quijano⁶, Emmanuel Pina-Jimenez³, Jesica Elena Mendieta-Wejebe⁷
¹Instituto Politecnico Nacional, UMAE Hospital de Especialidades-2° Piso CORCE Centro Médico Nacional Siglo XXI, IMSS, Pachuca, Hidalgo, México
²Instituto Politecnico Nacional, UMAE Hospital de Especialidades-2° Piso CORCE Centro Médico Nacional Siglo XXI, IMSS; Seccion de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, Plan de San Luis y Díaz Mirón, Pachuca, Hidalgo, México
³Unidad de Investigación Médica en Genética Humana, UMAE Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Doctores, CP, Pachuca, Hidalgo, México
⁴Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca, Hidalgo, México
⁵Sección de Estudios de Posgrado e Investigación Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340, México DF, México
⁶nstituto de Química de la Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, México DF, 04510, Ciudad de, México
⁷Seccion de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, Plan de San Luis y Díaz Mirón, Pachuca, Hidalgo, México

Abstract:

Background: Diabetes mellitus (DM) is a chronic disease characterized by high blood glucose levels resulting from insulin resistance or inadequate insulin secretion. In the world, DM is one of the most frequent non-contagious diseases that affect more than 371 million people. Objective: This study aimed to evaluate the antihyperglycemic properties of the ethanol extract, subsequent fractions, and farnesol obtained from the leaves of Annona diversifolia on alloxan-induced diabetic and normal mice. Materials and Methods: Bioassay-guided fractionation of the ethanol extract of the leaves of A. diversifolia (EELAd) was performed on alloxan-induced Type 2 diabetic and normoglycemic (NM) mice. Oral glucose tolerance test (OGTT), oral sucrose tolerance test (OSTT), and oral lactose tolerance test (OLTT) were performed in fast NM mice (FNM). Results: The EELAd, CHCl3 fraction, and farnesol induced a significant reduction of postprandial hyperglycemia in acute and subchronic tests using AITD mice. When EELAd, CHCl3 fraction, and farnesol were tested on NM in subchronic assays, these did not affect glycemic levels. In the case of acute test on NM, only CHCl3 fraction induced a hypoglycemic effect at 2 h after the treatment. OLTT and OSTT showed that the EELAd, CHCl3 fraction, and farnesol induced a significant reduction of hyperglycemia levels in FNM at 2 h after a lactose or sucrose load comparable to acarbose. In the case of OGTT was observed a significant reduction of hyperglycemia levels in FNM mice at 2 h after a glucose load comparable to canagliflozin. Conclusion: The EELAd and farnesol induced a significant reduction of postprandial hyperglycemia on AITD mice in acute and subchronic assays. Our results suggest that the control of postprandial hyperglycemia may be mediated by the regulation of absorption of glucose and inhibition of disaccharide digestion such as sucrose and lactose. Finally, the results explained the use of A. diversifolia in Mexican traditional medicine as an antihyperglycemic agent.