Background: Ultraviolet (UV) radiation causes photoaging in human skin. Phytochemicals from herbs have been used to treat various skin problems. Bioconversion of phytochemicals can increase efficacy and absorption rate by fermentation. Objective: The aim was to evaluate the anti-wrinkle of enriched-baicalein Scutellaria baicalensis (baicalin) was bioconverted by fermented with Leatiporus sulphureus (enriched-baicalein). Materials and Methods: Bioconversion of baicalein from baicalin were analyzed by high-performance liquid chromatography. The effect of enriched-baicalein on matrix metalloproteinase (MMP)-1 and procollagen production was examined in neonatal human dermal fibroblasts (HDFn) using ELISA, and then the effect of enriched-baicalein on wrinkle formation, skin thickness, and changes in collagen fibers were evaluated in hairless mice by histological analysis of their skin. Results: In vitro enriched-baicalein reduced UVB-induced MMP-1 production and promoted procollagen synthesis. In vivo, enrich-baicalein inhibited UVB-induced wrinkle formation, epidermal thickening, and damage to collagen fiber. Conclusions: These results suggest that enriched-baicalein has the potential to prevent wrinkle formation by inhibition of MMP-1 and promotion of Type-1 procollagen in HDFn, and by inhibition of skin thickness, and changes in collagen fibers in UVB-induced mice.