Antitumor effects of dammarane-type saponins from steamed Notoginseng

Fan He; Yan Ding; Chun Liang; Seok Bean Song; De-Qiang Dou; Gyu Yong Song; Young Ho Kim

Articles

Abstract

Pharmacognosy Magazine,2014,10,39,314-317.

DOI:10.4103/0973-1296.137372

Published:July 2014

Type:Original Article

Authors:

Author(s) affiliations:

Fan He¹, Yan Ding², Chun Liang³, Seok Bean Song⁴, De-Qiang Dou¹, Gyu Yong Song⁴, Young Ho Kim⁴
¹ Department of Pharmacognosy, Liaoning University of Traditional Chinese Medicine, Dalian, China
² Institute of Chemistry and Applications of Plant Resources, Dalian Polytechnic University, Dalian, China
³ Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine, Daejeon, Korea
⁴ College of Pharmacy, Chungnam National University, Daejeon, Korea

Abstract:

Six dammarane-type saponins were extracted from steamed Panax notoginseng. Their chemical structures were identified spectroscopically as ginsenosides Rh1 (1), Rg1 (2), 20 (S)-Rg3 (3), 20 (R)-Rg3 (4), Rb3 (5), and Rb1 (6). Compounds (0.1-10 μM) were tested for inhibition of tumor necrosis factor-α (TNF)-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) luciferase reporter activity using a human kidney 293T cell-based assay. Ginsenoside Rb3 (5) showed the most significant activity with an IC50 of 8.2 μM. This compound also inhibited the induction of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) messenger Ribonucleic acid (mRNA) in a dose-dependent manner after HepG2 cells had been treated with TNF-α (10 ng/mL).

Keywords:Antitumor, Dammarane-type ginsenoside, inhibitory activity, Panax notoginseng