Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid chromatography-diode array detection

Bo-Ra Yun; Jin Bae Weon; Jiwoo Lee; Min Rye Eom; Choong Je Ma

Articles

Abstract

Pharmacognosy Magazine,2014,10,38s,s256-s263.

DOI:10.4103/0973-1296.133267

Published:May 2014

Type:Original Article

Authors:

Author(s) affiliations:

Bo-Ra Yun¹, Jin Bae Weon¹, Jiwoo Lee¹, Min Rye Eom¹, Choong Je Ma²
¹ Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea
² Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University; Research Institute of Biotechnology, Kangwon National University, Chuncheon, Republic of Korea

Abstract:

Background: Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus. Objective: A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- hydroxymethylfurfural, mangiferin, paeoniflorin, nodakenin, naringin, hesperidin, decursinol, berberine, glycyrrhizin, atractylenolide III and decursin, in JEGH. Materials and Methods: The separation was conducted on Shishedo C ₁₈ column with gradient elution of 0.1% trifluoroacetic acid-acetonitrile. Detection of wavelength was set at 205, 250, 280 and 330 nm. Results: The developed analysis showed a good linearity (R² >0.9997). The range of limit of detection and limit of quantification were observed from 0.04 to 0.43 and from 0.11 to 1.30, respectively. The intra- and inter-day test relative standard deviations (RSD) were less than 3% and the accuracy was 95.98-108.44%. The recoveries were between 92.75% and 109.19% and RSD range of recoveries was measured from 0.52% to 2.78%. Conclusion: This HPLC-DAD method can be successfully applied for simultaneous determination of 11 major compounds in JEGH samples.