Protective effects on myelosuppression mice treated by three different classic chinese medicine formulae

An-bin Zhao; Bin YU; Xian-Lin WU; Ke-Jian Cao; En-Qing LI; Qing-Mei LI; Xiao-Yin Chen

Articles

Abstract

Pharmacognosy Magazine,2011,7,26,133-140.

DOI:10.4103/0973-1296.80671

Published:May 2011

Type:Original Article

Authors:

Author(s) affiliations:

An-bin Zhao¹, Bin YU², Xian-Lin WU², Ke-Jian Cao³, En-Qing LI², Qing-Mei LI⁴, Xiao-Yin Chen²
¹Department of Internal Medicine, Xinzheng Chinese Medical Hospital, Zhengzhou, China
²Department of Traditional Chinese Medicine, Medical College, Jinan University, Guangzhou, China
³Department of Traditional Chinese Medicine, School of Chinese Medicine, The University of Hong Kong, Hong Kong
⁴Department of Laboratory, Guangzhou Red Cross Hospital, Guangzhou, China

Abstract:

Background: In order to observe the protective therapeutic action and mechanism of Liuwei Dihuang Decoction, Buzhong Yiqi Decoction, and Compound Danshen Decoction on Myelosuppression induced by cyclophosphamide. Materials and Methods: The mice model was established by intraperitoneal injected with 100 mg/kg cyclophosphamide by human and mice dose conversion on the 9 ^th , 11 ^th , 13 ^th days during the experiment. Flow cytometry (FCM) was used for detecting the number of cells and investigating bone marrow cell cycles. Spleen was taken out and the mRNA expression level of thrombopoietin (TPO) and c-Mpl were detected by Q-PCR, and c-Mpl in spleen in order to discuss the mechanism of myelosuppression and the protective effects of traditional Chinese medicine. Results: Both Liuwei Dihuang Decoction Group and Buzhong Yiqi Decoction Group can accelerate bone marrow hematopoietic stem progenitor cells (HSPCs) in marrow-suppressed mice and enhance cell proliferation by promoting cell cycles from G0/G1 phase to access into S, G2/M phase. And at the same time these Chinese decoctions can increase the mRNA expression level of TPO and c-Mpl in spleen. Conclusion: Researched showed that Chinese formula take effect by affecting these genes on myelosuppressed mice.