Orthogonal test design for optimization of the extraction of polysaccharide from Paeonia sinjiangensis K.Y. Pan

Shuge Tian; Xiaoying Zhou; Haiyan Gong; Xiuming Ma; Fan Zhang

Articles

Abstract

Pharmacognosy Magazine,2011,7,25,4-8.

DOI:10.4103/0973-1296.75874

Published:January 2011

Type:Original Article

Authors:

Author(s) affiliations:

Shuge Tian¹, Xiaoying Zhou², Haiyan Gong³, Xiuming Ma³, Fan Zhang¹
¹Xinjiang Key Laboratory of Famous Prescription and Science of Formulas; College of TCM, XinJiang Medical University, Urumqi - 830 011, XinJiang, China
²College of Pharmacy, XinJiang Medical University, Urumqi - 830 011, XinJiang, China
³College of Basic Medical, XinJiang Medical University, Urumqi - 830 011, XinJiang, China

Abstract:

Background: Paeonia sinjiangensis K.Y. Pan is a perennial herb belonging to the ranunculaceae family and it is one of the most important crude drugs in traditional Chinese medicine. In this article, Paeonia sinjiangensis K.Y. Pan rich in polysaccharide is used as an experimental material. Materials and Method: Study the effects of proportion, temperature, times and time taken for the extraction yield of polysaccharide through a single-factor exploration. Then, through an orthogonal experiment (L₉(3)⁴), it was investigated to get the best extraction conditions. Results: The results showed that the ratio of solvent to raw material, number of extractions and duration of extraction were the main variables that influenced the yields of extracts. The separation procedure of precipitation with alcohol and the purification from the removing proteins were deeply analyzed. Meanwhile the contents of polysaccharide were determined by anthrone colorimetry. Conclusion: The highest yield was obtained when the ratio of solvent to raw material, number of extractions, and duration of extraction were 8:1, 2, and 1.5 h, respectively. The content of soluble polysaccharide is 51.57%.