The present study was undertaken to evaluate antistress, annxiolytic and hypnotic activity of Vedic calm, a polyherbal formulation comprising of Bacopa monnieri, Centella asiatica, Evolvulus alsinides and many other related plants extracts, all of which are classified in ayurveda as rasayanas which are reported to promote physical and mental health and also possess depressant activity. Antistress activity was evaluated by cold immobilization induced stress for 10 days in rats using Withania somnifera (100 mg/kg) as reference standard. Antianxiety activity was evaluated by elevated plus maze and light and dark box in acute study and elevated plus maze in chronic study of 10 days in rats, using diazepam (2 mg/kg) as reference standard. Hypnotic activity was evaluated by potentiation of thiopental induced sleeping time in mice. Stress was evidenced by occurrence of gastric ulcer, elevation of adrenals weight, liver weight, serum glucose, AST, ALT, cholesterol, WBC and reduction of spleen weight. Vedic calm pretreatment in rats with doses of 135 mg/kg, 270 mg/kg p.o. and standard drug Withania somnifera significantly reversed all the changes those were due to stress. The Vedic calm at 270 mg/kg significantly increased the time spent and number of entries into open arms in elevated plus maze and increased the time spent and number of entries into light box in light and dark box model. The Vedic calm at a dose of 390 mg/kg significantly potentiated duration of thiopental induced sleeping time in mice. The Vedic calm showed significant antistress, anxiolytic and hypnotic activity.