Determination and tissue distribution comparisons of atractylodin after oral administration of crude and processed atractylodes rhizome

Background: Atractylodis rhizoma is one of the most often used drugs in traditional Chinese medicine. Stir frying with wheat bran is the most common processing method. To clarify the principle of processing, an experiment was carried out to compare the tissue distribution of typical constituent after oral administration of raw A. rhizoma and processed ones. Objective: To compare the tissues distribution of atractylodin after oral administration of raw and processed A. rhizoma and clarify the processing principle of A. rhizoma. Materials and Methods: High-performance liquid chromatogram with ultraviolet detection was developed and validated for the determination of atractylodin in rat tissues. Results: The atractylodin in raw and processed A. rhizoma was distributed in all tissues involved in this study. Conclusions: The concentration of atractylodin in it is the highest in the stomach and small intestine.