Antioxidant and nephroprotective activities of the extract and fractions of Homonoia riparia Lour

Seena kanniparambil Xavier; Shoja Muhammed Haneefa; Devkar Raviraj Anand; Picheswara Rao polo; Rajalekshmi Maheshwari; Chandrashekara Shastry Shreedhara; Manganahalli Manjunath Setty

Articles

Abstract

Pharmacognosy Magazine,2017,13,49,25-30.

DOI:10.4103/0973-1296.197645

Published:January 2017

Type:Original Article

Authors:

Author(s) affiliations:

Seena kanniparambil Xavier¹, Shoja Muhammed Haneefa², Devkar Raviraj Anand¹, Picheswara Rao polo¹, Rajalekshmi Maheshwari¹, Chandrashekara Shastry Shreedhara¹, Manganahalli Manjunath Setty¹
¹Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India
²Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India

Abstract:

Background: Homonoia riparia is a plant, which is widely used in the indigenous system of medicine for the treatment of urolithiasis, renal disorders and inflammatory conditions. This is the first report on the antioxidant and nephroprotective activities of whole plant of H. riparia. Objective: The present study aims at investigating the in vitro antioxidant and nephroprotective activity of the methanol extract and its different fractions of H. riparia. Methods: Petroleum ether (HRPE), Ethyl acetate (HREA), Butanol (HRBU), aqueous fractions (HRAQ) were prepared from the crude methanol extract of H. riparia (HRM) using liquid partitioning. Total phenolic content, flavonoid content and antioxidant activity assay were performed according to suitable methods. Nephroprotective activities were evaluated by MTT assay using Human Embryonic Kidney cells against cisplatin induced toxicity. Quantification of gallic acid was performed using validated HPTLC method. Results: The studies showed that extract and fractions possess significant nephroprotective activity against cisplatin induced renal toxicity. All the extracts/fractions of whole plant of Homonoia riparia was found to be significantly reducing cisplatin induced toxicity (< 0.05). The highest activity was observed with HRBU and HRAQ with a percentage viability of 293.09 ± 4.3 and 345.07 ± 3.2 at a concentration of 200 µg/ml. Gallic acid was detected in the HRM/fractions using HPTLC.