Protein expression patterns in hek-Blue™ - Cells treated with Clinacanthus nutans extracts

Shun-Kai Yang; Teik-Kee Leo; Chun-Wai Mai; Swee-Yee Chin; Dhilia Udie Lamasudin; Swee-Hua-Erin Lim; Ngai-Paing Tan; Kok-Song Lai

Articles

Abstract

Pharmacognosy Magazine,2020,16,70,431-434.

DOI:10.4103/pm.pm_281_19

Published:August 2020

Type:Original Article

Authors:

Author(s) affiliations:

Shun-Kai Yang¹, Teik-Kee Leo², Chun-Wai Mai³, Swee-Yee Chin⁴, Dhilia Udie Lamasudin¹, Swee-Hua-Erin Lim⁵, Ngai-Paing Tan⁶, Kok-Song Lai⁵
¹ Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia
² Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia
³ Department of Pharmaceutical Chemistry, School of Pharmacy; Centre for Cancer and Stem Cells Research, Institute for Research, Development and Innovation, International Medical University, Kuala Lumpur, Malaysia
⁴ Department of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia
⁵ Health Sciences Division, Abu Dhabi Women's College, Higher Colleges of Technology, Abu Dhabi, United Arab Emirates
⁶ Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia

Abstract:

Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process.