Cytotoxicity, protein kinase inhibitory activity, and docking studies of secondary metabolites isolated from Brownea grandiceps Jacq

Ehab M Mostafa; Arafa Musa; Mohamed A Abdelgawad; Ehab A Ragab

Articles

Abstract

Pharmacognosy Magazine ,2019,15,63,438-442.

DOI:10.4103/pm.pm_35_19

Published:May 2019

Type:Original Article

Authors:

Author(s) affiliations:

Ehab M Mostafa¹, Arafa Musa¹, Mohamed A Abdelgawad², Ehab A Ragab³
¹Department of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka, Aljouf, KSA; Department of Pharmacognosy, Faculty of Pharmacy, Al Azhar University, Cairo, Egypt
²Department of Pharmaceutical Chemistry, College of Pharmacy, Jouf University, Sakaka, Aljouf, KSA; Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy Beni-Suef University, Beni Suef, Egypt
³Department of Pharmacognosy, Faculty of Pharmacy, Al Azhar University, Cairo, Egypt

Abstract:

Background: Numerous kinases are excessively secreted in cancer. Consequently, inhibition of kinase enzymes has a basic role in the treatment of cancer through suppression or prevention of cancer cell multiplication. Due to its kinase inhibitory activity, flavonoids are expected to be of great importance in the discovery of new anticancer drugs. Based on the chemotaxonomic relationship among the plant genera, Brownea grandiceps is expected to be rich in flavonoids. Objective: The objective was to study the cytotoxicity, kinase inhibitory activity, and docking of the isolated metabolites. Materials and Methods: Ultraviolet, nuclear magnetic resonance, and mass spectroscopy were used for the identification and confirmation of the active metabolites. Ethyl acetate extract of B. grandiceps was chromatographed. Cytotoxicity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), protein kinase (PK) inhibitory assays, and molecular operating environment (using Molecular Operating Environment [MOE], 2016.08) were performed. Results: Catechin (1), quercitrin (2), trans-taxifolin-3-O-α-L-rhamnopyranoside (3), 13R-hydroxy-9Z, 11E-octadecadienoic acid (4), and 13R-hydroxy-9Z, 11E-, 15Z-octadecatrienoic acid (5) were isolated and identified. Quercitrin (2) exhibited the highest cytotoxicity against MCF-7 (inhibitory concentration for 50% [IC50]: 4.24 μM) and moderate cytotoxicity against HepG2 cell (IC50: 29.58 μM). Others showed moderate cytotoxicity; compounds 1–3 were tested against Aurora B, CDK4/cyclin D1, COT, IGF1-R, and FAK PKs, where quercitrin showed the highest inhibitory activity against Aurora B and CDK4/Cyclin D1 (IC50: 4.78 and 3.22 μM). Docking of quercitrin against CDK4/Cyclin D1 confirmed its cytotoxic profile. Conclusion: The metabolites were first isolated from B. grandiceps. The mechanism of action against kinases enzyme was established and confirmed by docking studies of quercitrin at CDK4/Cyclin D1 using MOE program.