Molecular authentication of the traditional medicinal plant Peucedanum praeruptorum and its substitutes and adulterants by dna - barcoding technique

Jing Zhou; Wencai Wang; Mengqi Liu; Zhenwen Liu

Articles

Abstract

Pharmacognosy Magazine,2014,10,40,385-390.

DOI:10.4103/0973-1296.141754

Published:September 2014

Type:Original Article

Authors:

Author(s) affiliations:

Jing Zhou¹, Wencai Wang², Mengqi Liu³, Zhenwen Liu⁴
¹ School of Pharmaceutical Sciences & Yunnan Key Laboratory of Pharmacology for Nature Products, Kunming Medical University,Kunming 650500, China
² Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
³ Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China
⁴ Key Laboratory of Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China

Abstract:

Background: Peucedanum praeruptorum L., a traditional Chinese medicine known as Qian-hu, is commonly used for dispelling wind-heat and expectorant and loss of energy. However, due to similar morphological characters and high market demand, there are many substitutes and adulterants of P. praeruptorum. DNA barcoding is an approach to identify species based on sequences from a short, standardized DNA region. Objective: To authenticate P. praeruptorum from its substitutes and adulterants. Materials and Methods: The differential identification of P. praeruptorum and 13 regional substitutes and 23 adulterants was investigated by means of DNA sequence analysis of internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA), a bootstrap neighbor-joining (NJ) tree according to Kimura's 2-parameter method was also calculated. Results: The data showed that P. praeruptorum, its substitutes and adulterants could be easily distinguished at the DNA level, while almost all species were well resolved, and successfully identified on the NJ tree. Conclusion: The ITS sequence can be used for the identification of P. praeruptorum and to distinguish it from common substitutes and adulterants.