Close
  Indian J Med Microbiol
 

Figure 4: The effects of PR extract on cell caspase 3 activity, ROS formation, and mitochondrial membrane potential (MMP). For caspase 3 activity, the cancer cells were treated with PR extract (0-500 mg/mL) and measured the caspase 3 activity by Caspase 3 assay kit (a) For ROS formation, cells were treated with PR extract (0-1000 mg/mL) plus DHE-fluorescent probe for 90 min, and then measured ROS formation by spectrophotometer (b) For JC-1 method, cells were treated with PR extract (0-500 mg/mL) for 24 h, stained with JC-1 for 30 min, and then measured the fluorescent intensity (c) *p < 0.05 vs. control groups

Figure 4: The effects of PR extract on cell caspase 3 activity, ROS formation, and mitochondrial membrane potential (MMP). For caspase 3 activity, the cancer cells were treated with PR extract (0-500 mg/mL) and measured the caspase 3 activity by Caspase 3 assay kit (a) For ROS formation, cells were treated with PR extract (0-1000 mg/mL) plus DHE-fluorescent probe for 90 min, and then measured ROS formation by spectrophotometer (b) For JC-1 method, cells were treated with PR extract (0-500 mg/mL) for 24 h, stained with JC-1 for 30 min, and then measured the fluorescent intensity (c) <i>*p</i> < 0.05 vs. control groups