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  Indian J Med Microbiol
 

Figure 2: Extracts of sample S41 inhibits receptor activator of nuclear factor NF-kB ligand-induced osteoclastogenesis in bone marrow-derived macrophages and RAW264.7 cells. (a) RAW264.7 cells were cultured into 96 well-plates under stimulation of receptor activator of nuclear factor NF-kB ligand (100 ng/ml) with or without indicated concentrations of plant extract S41 for 4 days, then the cells were stained for TRAP. The quantities of TRAP-positive multinucleated (>3 nuclei) osteoclasts were determined following image capture (×40). Data are presented as the mean ± standard error (*P < 0.01, vs. vehicle-treated control; n = 3). (b) Effects of S41 on receptor activator of nuclear factor NF-kB ligand-induced osteoclastogenesis in bone marrow-derived macrophages. Bone marrow-derived macrophages were seeded into 96 well-plates in the presence of M-CSF (30 ng/ml) and receptor activator of nuclear factor NF-kB ligand (100 ng/ml) with or without indicated concentrations of the plant extract S41 and then the cells were stained for TRAP. The quantities of TRAP-positive multinucleated (>3 nuclei) osteoclasts were determined following image capture (×40). Data are presented as the mean ± SE (*P < 0.01, vs. vehicle-treated control; n = 3)

Figure 2: Extracts of sample S41 inhibits receptor activator of nuclear factor NF-kB ligand-induced osteoclastogenesis in bone marrow-derived macrophages and RAW264.7 cells. (a) RAW264.7 cells were cultured into 96 well-plates under stimulation of receptor activator of nuclear factor NF-kB ligand (100 ng/ml) with or without indicated concentrations of plant extract S41 for 4 days, then the cells were stained for TRAP. The quantities of TRAP-positive multinucleated (>3 nuclei) osteoclasts were determined following image capture (×40). Data are presented as the mean ± standard error (*<i>P </i>< 0.01, vs. vehicle-treated control; <i>n</i> = 3). (b) Effects of S41 on receptor activator of nuclear factor NF-kB ligand-induced osteoclastogenesis in bone marrow-derived macrophages. Bone marrow-derived macrophages were seeded into 96 well-plates in the presence of M-CSF (30 ng/ml) and receptor activator of nuclear factor NF-kB ligand (100 ng/ml) with or without indicated concentrations of the plant extract S41 and then the cells were stained for TRAP. The quantities of TRAP-positive multinucleated (>3 nuclei) osteoclasts were determined following image capture (×40). Data are presented as the mean ± SE (*<i>P</i> < 0.01, vs. vehicle-treated control;<i> n</i> = 3)